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Merck
CN

B8026

Bromophenol Blue sodium salt

For molecular biology, powder

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
Molecular Weight:
691.94
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
252-170-2
MDL number:
Beilstein/REAXYS Number:
6995701
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Product Name

Bromophenol Blue sodium salt, Molecular Biology, suitable for electrophoresis

grade

Molecular Biology

Quality Level

form

powder

technique(s)

electrophoresis: suitable

visual transition interval

3.0-4.6, yellow to blue

ε (extinction coefficient)

≥150000 at 597-603 nm in H2O, ≥17000 at 250-256 nm in H2O, ≥23000 at 306-312 nm in H2O

suitability

suitable for (Suitable For Use as a Tracking Dye For Electrophoresis in Alkaline and Neutral Buffers)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

General description

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

Application

Bromophenol Blue sodium salt has been used in the buffer solution to differentiate dead and living cells. It has also been used as a constituent in gel loading buffer.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction
Salomon S, et al.
Electrophoresis, 32(12), 1508-1514 (2011)
Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography
Speziale P, et al.
Nature Protocols, 3(3), 525-525 (2008)
Loss of osmoregulation of OmpC porin occurs if Escherichia coli are deficient in cAMP receptor protein
Joo SH, et al.
Journal of Experimental Microbiology and Immunology, 17, 78-82 (2013)



Global Trade Item Number

SKUGTIN
B8026-25G04061833442401
B8026-5G04061833442418