CHAPS hydrate

CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution.

Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

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