D9564
DAPI, dilactate
≥98% purity (HPLC), powder
Synonym(s):
4′,6-Diamidino-2-phenyindole, dilactate
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About This Item
Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Product Name
DAPI, dilactate, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
technique(s)
titration: suitable
color
yellow
solubility
H2O: 20 mg/mL
ε (extinction coefficient)
28.00-32.50 at 345-351 nm in methanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
InChI key
LCAUPANIXBMNIA-UHFFFAOYSA-N
General description
DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.
Application
DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.
Biochem/physiol Actions
A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Silvia S Kang et al.
The Journal of experimental medicine, 208(4), 747-759 (2011-04-06)
After virus infection, cytotoxic T lymphocytes (CTLs) divide rapidly to eradicate the pathogen and prevent the establishment of persistence. The magnitude of an antiviral CTL response is thought to be controlled by the initiation of a cell cycle program within
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
Vigano F, et al.
Nature Neuroscience, 16, 1370-1372 (2013)
Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Muller glial cells.
Chavira-Suarez E, et al.
Biochemical and Biophysical Research Communications, 404, 678-683 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D9564-10MG | 04061833590867 |