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E7889

Ethylenediaminetetraacetic acid disodium salt solution

Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS Number:
139-33-3
Molecular Weight:
336.21
MDL number:
MFCD00070672
Beilstein/REAXYS Number:
3822669
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
24894660
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grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

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pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H373

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL5409
BCCG6590
BCCG1836
BCCG0945
BCCF9230

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Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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Global Trade Item Number

SKUGTIN
E7889-100ML04061838255334
E7889-1L04061838110152