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E9379

Stains-All

Name: Stains-All
Brand: SIAL

~95% purity, powder

Synonym(s):

1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine

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About This Item

Empirical Formula (Hill Notation):

C₃₀H₂₇BrN₂S₂

CAS Number:

7423-31-6

Molecular Weight:

559.58

NACRES:

NA.47

PubChem Substance ID:

24894694

UNSPSC Code:

12171500

EC Number:

231-047-7

MDL number:

MFCD00078788

Beilstein/REAXYS Number:

3865213

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Properties

Product Name

Stains-All, ~95%

assay

~95%

Quality Level

200

form

powder

technique(s)

microbe id | staining: suitable

solubility

chloroform: 40 mg (PLUS 4.0 ml)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC

InChI

1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

MPBRYMWMMKKRGC-UHFFFAOYSA-M

Description

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Disclaimer

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Rehm H

Protein Biochemistry and Proteomics. The experimenter series (2006)

Kinetics of duplex formation for individual DNA strands within a single protein nanopore.

Howorka S, et al.

Proceedings of the National Academy of Sciences of the USA, 98, 12996-12996 (2001)

A comparison of the sensitivity, specificity, and molecular weight accuracy of three different commercially available Hyaluronan ELISA-like assays.

Haserodt S, et al.

Glycobiology, 21, 175-175 (2011)

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Global Trade Item Number

SKU	GTIN
E9379-5G	04061832342801
E9379-1G	04061822703711