F5415
Ficoll® solution
Type 400, 20% in H2O
Synonym(s):
Ready-to-use density gradient media
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About This Item
sterility
0.2 μm filtered
Quality Level
type
Type 400
form
liquid
concentration
20% in H2O
technique(s)
titration: suitable
color
clear colorless
solubility
water: soluble
density
1.055-1.075 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
General description
Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.
Application
Ficoll® has been used in
- Isolation of cord blood mononuclear cells
- Isolation of CD133 negative cells
- Isolation and expansion of circulating endothelial progenitor cells.
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F5415-25ML | 04061833618233 |
| F5415-50ML | 04061833618240 |