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F7258

Fast Green FCF

certified by the BSC

Synonym(s):

Food green 3

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About This Item

Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
EC Number:
219-091-5
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894936
MDL number:
MFCD00013053
Beilstein/REAXYS Number:
5718212
Colour Index Number:
42053
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agency

certified by the BSC

Quality Level

200

form

powder

composition

dye content, ≥90%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

Application

Fast Green FCF has been used:
  • along with safranin for the staining of nodule sections
  • in the collagen deposit staining procedure
  • for the staining of protein and starch
  • as a dye to detect and inject DNA solution in chicken embryo
Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H335,H341

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBP1255
SHBN5409
SHBM6976
SHBL4767
MKCG7515

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Combination of in ovo electroporation and time-lapse imaging to study migrational events in chicken embryos.
Masyuk M et al.
Developmental Dynamics, 243, 690-690 (2014)
Micro-morphology of common bean (Phaseolus vulgaris L.) nodules undergoing senescence.
Fernandez-Luque?o F et al.
Acta Physiologiae Plantarum / Polish Academy of Sciences, Committee of Plant Physiology Genetics and Breeding, 30, 545-552 (2008)
Jill J Weyers et al.
PloS one, 15(1), e0227780-e0227780 (2020-01-17)
The engraftment of human stem cell-derived cardiomyocytes (hSC-CMs) is a promising treatment for remuscularizing the heart wall post-infarction, but it is plagued by low survival of transplanted cells. We hypothesize that this low survival rate is due to continued ischemia
View All Related Papers

Global Trade Item Number

SKUGTIN
F7258-25G04061833619421