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N5285

Neomycin trisulfate salt hydrate

Name: Neomycin trisulfate salt hydrate
Brand: SIAL

meets USP testing specifications, powder

Synonym(s):

Framycetin sulfate, Neomycin Sulfate

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About This Item

Empirical Formula (Hill Notation):

C₂₃H₄₆N₆O₁₃ · 3H₂SO₄ · xH₂O

CAS Number:

1405-10-3

Molecular Weight:

908.88 (anhydrous basis)

PubChem Substance ID:

24897730

UNSPSC Code:

51281659

NACRES:

NA.76

EC Number:

215-773-1

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Properties

Quality Level

200

agency

USP/NF, meets USP testing specifications

form

powder

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

application(s)

pharmaceutical (small molecule)

mode of action

protein synthesis | interferes

SMILES string

O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O

InChI

1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1

InChI key

WHAGUNPVKDUVFV-QGTTWHFQSA-N

Description

General description

Chemical structure: aminoglycoside

Stock solutions should be filter sterilized and stored at 2-8 °C. Stable at 37 °C for 5 days.

Application

Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins. It is recommended for use in cell culture applications at 50 mg/L.

Biochem/physiol Actions

Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.

Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.

Preparation Note

Neomycin sulfate is soluble in H₂O at 50 mg/mL, yielding a clear solution.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

Disclaimer

Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

涉药品监管产品

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Obesity induces gut microbiota alterations and augments acute graft-versus-host disease after allogeneic stem cell transplantation.

Lam T Khuat et al.

Science translational medicine, 12(571) (2020-11-27)

The efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is limited by acute and chronic graft-versus-host disease (GVHD). The impact of obesity on allo-HSCT outcomes is poorly understood. Here, we report that obesity had a negative and selective impact on

Determination of neomycin and related substances in pharmaceutical preparations by reversed-phase high performance liquid chromatography with mass spectrometry and charged aerosol detection.

K Stypulkowska et al.

Journal of pharmaceutical and biomedical analysis, 76, 207-214 (2013-01-23)

A new, simple and repeatable liquid chromatographic method with charged aerosol detection (LC-CAD) for determination of neomycin and related substances has been developed. Analysis of neomycin or other aminoglycosides is problematic due to a lack of chromophores. Universal response of

Outcome of a second patch test reading of TRUE Tests® on D6/7.

Jakob Torp Madsen et al.

Contact dermatitis, 68(2), 94-97 (2013-01-08)

Background. Two readings of patch test reactions are recommended. Objectives. To evaluate the outcome of a second patch test reading of TRUE Test® allergens on D6/7 in relation to negative or doubtful reactions on D3/4. Methods. This was a retrospective

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Global Trade Item Number

SKU	GTIN
N5285-100G	04061834116936
N5285-25G	04061833622858