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Merck
CN

P5504

Potassium phosphate dibasic trihydrate

ReagentPlus®, ≥99.0%

Synonym(s):

Dipotassium hydrogen phosphate trihydrate, Dipotassium phosphate, di-Potassium hydrogen phosphate trihydrate

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About This Item

Linear Formula:
K2HPO4 · 3H2O
CAS Number:
Molecular Weight:
228.22
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
231-834-5
MDL number:
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Quality Level

product line

ReagentPlus®

assay

≥99.0%

form

powder or crystals

pH

9.2-9.4 (25 °C, 50 g/L)

solubility

water: 100 mg/mL, clear, colorless

storage temp.

room temp

SMILES string

O.O.O.[K+].[K+].OP([O-])([O-])=O

InChI

1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2

InChI key

XQGPKZUNMMFTAL-UHFFFAOYSA-L

General description

Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.

Application

Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

在测定超氧化物歧化酶活性时,使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50%。

Enzymatic Assay of Superoxide Dismutase

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Global Trade Item Number

SKUGTIN
P5504-100G04061835547760
P5504-5KG04061834391791
P5504-10KG04061833623282
P5504-1KG04061835547777
P5504-500G04061835547784