PPB002
Tris Buffered Saline
powder, pH 8.0, pHast Pack™, with 0.05% Tween® 20
Synonym(s):
Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
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About This Item
UNSPSC Code:
41105323
NACRES:
NA.21
Product Name
Tris Buffered Saline, with 0.05% Tween® 20, pH 8.0, powder, pHast Pack™
product line
pHast Pack™
form
powder
storage condition
(Tightly closed. Dry.)
impurities
≤5 ppm Heavy Metals (lead)
color
white
pH
7.8—8.2
solubility
soluble, clear to hazy, colorless
cation traces
Fe: ≤10 ppm
foreign activity
DNase, none detected, Endonuclease , Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected
storage temp.
room temp
General description
Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.
Application
Suitable for use as a wash buffer in:
- Western blot
- ELISA
- Immunohistochemistry
Features and Benefits
- Ready-to-use buffer requires no measurments or pH adjustments
- Saves time, money and effort to get your assays started fast
- Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
- Qualified for use in gel electrophoreis and other molecular biology assays
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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