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Merck
CN

04276

Sodium phosphate dibasic

puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
EC Number:
231-448-7
NACRES:
NA.25
MDL number:
Assay:
98-100.5% (calc. to the dried substance)
Form:
powder
Solubility:
H2O: soluble 8 parts, H2O: very soluble (Hot), alcohol: insoluble
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Quality Level

agency

meets analytical specifications of BP, meets analytical specifications of Ph. Eur., meets analytical specifications of USP

grade

puriss.

assay

98-100.5% (calc. to the dried substance)

form

powder

quality

meets analytical specification of Ph. Eur., BP, USP, FCC, E 339

impurities

reducing matter, complies, residual solvents, complies, ≤0.001% heavy metals (as Pb), ≤0.002% ammonium (NH4), ≤0.2% water-insoluble matter, ≤1% NaH2PO4 (calc. to dried subst.), ≤2.0% pyrophosphate (as Na4P2O7)

loss

≤1% loss on drying, 105°C, 4h, ≤1% loss on drying, 130 °C

pH

8.4-9.6 (20 °C, 1%)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: soluble 8 parts, H2O: very soluble (Hot), alcohol: insoluble

anion traces

chloride (Cl-): ≤50 mg/kg, fluoride (F-): ≤10 mg/kg, sulfate (SO42-): ≤200 mg/kg

cation traces

As: ≤2 mg/kg, Cd: ≤1 mg/kg, Cu: ≤10 mg/kg, Fe: ≤20 mg/kg, Hg: ≤1 mg/kg, Pb: ≤4 mg/kg, Zn: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

Application

Sodium phosphate dibasic has been used for preparing phosphate buffer which in turn is utilized in X-gal staining.

Biochem/physiol Actions

Sodium phosphate dibasic is an important component of running buffer of denaturing gel electrophoresis. It induces glomerular calcification as well as other nephrotic syndrome in Sprague-Dawley rats.


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Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Noriko Tsuchiya et al.
Toxicologic pathology, 32(4), 408-412 (2004-06-19)
To elucidate the nephrotoxicity of phosphate, dibasic sodium phosphate solution was given to Sprague-Dawley rats by daily bolus intravenous administration at concentrations of 0, 1, 25, 250, or 360 mM (0, 1, 28, 284, or 408 mg/kg Na2HPO4) for 14
B Duvillié et al.
Endocrinology, 143(4), 1530-1537 (2002-03-19)
The targeted disruption of the two nonallelic insulin genes in mouse was reported previously to result in intrauterine growth retardation, severe diabetes immediately after suckling, and death within 48 h of birth. We have further used these animals to investigate
Noriko Tsuchiya et al.
Toxicologic pathology, 37(3), 270-279 (2009-02-27)
Sprague-Dawley rats received once daily tail-vein injections of 360 mM dibasic sodium phosphate solution at 8 mL/kg for fourteen or twenty-eight days. Clinical examination revealed persistent proteinuria from three days after the first dosing and thereafter severe proteinuria from eight



Global Trade Item Number

SKUGTIN
301515-10G04061832641904
301515-50G04061832641911
04276-4X5KG04061838629401
04276-1KG04061838629395
04276-25KG04061833616574
04276-5KG04061838629418
04276-6X1KG04061833347515