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Merck
CN

270733

Water

suitable for HPLC

Synonym(s):

Aqua, Dihydrogen oxide

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
UNSPSC Code:
12190000
NACRES:
NA.21
PubChem Substance ID:
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
Grade:
HPLC grade
Technique(s):
HPLC: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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grade

HPLC grade

vapor density

<1 (vs air)

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01, λ: 210 nm Amax: 0.01, λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.

Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.

Packaging

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.
Discover LiChropur reagents ideal for HPLC or LC-MS analysis


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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocols

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.

Articles

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S Bekesova et al.
Journal of proteomics, 75(7), 2216-2224 (2012-02-14)
N-glycosylation of proteins provides a rich source of information on liver disease progression because majority of serum glycoproteins, with the exception of immunoglobulins, are secreted by the liver. In this report, we present results of an optimized workflow for MALDI-TOF
Manolis Mandalakis et al.
Journal of chromatography. A, 1217(1), 143-150 (2009-12-08)
The analysis of amino acids by gas chromatography mass spectrometry (GC-MS) after their derivatization with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide was investigated as an alternative approach for the determination of free (FAA) and combined amino acids (CAA) in aerosols. This technique showed excellent linearity
Martin Fischnaller et al.
Analytica chimica acta, 761, 92-101 (2013-01-15)
Phosphorylation of proteins is an important cellular regulatory process. The analysis of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of phosphorylated species. Mass spectrometry (MS) of phosphopeptides obtained from tryptic protein digests is



Global Trade Item Number

SKUGTIN
270733-20L04061837478369
270733-4X4L04061837478383
270733-6X1L04061826142387
270733-18L04061826142370
270733-1L04061837478352
270733-4L04061837478376
270733-50L04061837285271