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03978

Methylene Blue solution

concentrate according to Ehrlich, concentrated, aqueous solution

Synonym(s):

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
NACRES:
NA.47
PubChem Substance ID:
329747853
UNSPSC Code:
12171500
MDL number:
MFCD00012111
Beilstein/REAXYS Number:
3641570
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Quality Level

100

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

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wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG5780
BCCF7036
BCCD6957
BCCC4840
BCCB5305

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Reprogramming human amniotic fluid stem cells to functional pluripotency by manipulation of culture conditions
Moschidou D and Guillot PV
National Protocol Exchange (2012)
Pathogen reduction technology treatment of platelets, plasma and whole blood using riboflavin and UV light
Transfusion Medicine and Hemotherapy (2011)
CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra
Cheng An-Chieh, et al.
Testing, 12(10), e0186780-e0186780 (2017)
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Global Trade Item Number

SKUGTIN
PHR2262-100MG04061841319634
214515-50G04061838773128
52245-25MG04061832547824
03978-250ML04061838628572