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Merck
CN

12103C

Fetal Bovine Serum

USA origin, suitable for cell culture

Synonym(s):

Fetal Calf Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Shipped in:
dry ice
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biological source

bovine fetus

Quality Level

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

Fetal Bovine Serum was used in different types of cell culture as a supplement, such as the following:
  • PC12 cells grown in Dulbecco′s modified Eagle′s medium supplemented with Fetal Bovine Serum along with other components.
  • HeLa cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing Fetal Bovine Serum along with other components.
  • BaF3 Cell Culture (cells were maintained in RPMI 1640 medium supplemented with Fetal Bovine Serum along with other components).
  • Primary hippocampal cell cultures (cells were maintained in Eagle′s minimum essential medium and supplemented with heat-inactivated fetal bovine serum along with other components).
  • HeLa, Cos1 and Cos7 cells were cultured in Dulbecco modified Eagle medium (DMEM) with fetal bovine serum along with other components.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.

This product is for further manufacturing use. THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions



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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

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Protocols

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

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Global Trade Item Number

SKUGTIN
12103C-1L04065266960594
12103C-500ML04061838251954
12103C-1000ML04061838261434
12103C-100ML04061838717252