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Merck
CN

14879

BIS-TRIS

BioUltra, ≥99.0% (NT)

Synonym(s):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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About This Item

Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:
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product line

BioUltra

Quality Level

assay

≥99.0% (NT)

form

powder

impurities

insoluble matter, passes filter test

ign. residue

≤0.3%

loss

≤0.5% loss on drying, 20 °C (HV)

pH

9.5-11.0 (25 °C, 1 M in H2O)

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

absorption

cut-off at 270 nm in H2O at 1 M

λ

1 M in H2O

UV absorption

λ: 280 nm Amax: 0.1

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

Application

BIS-TRIS can be used to study biological buffers. BIS-TRIS has been used in a study that synthesized ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. BIS-TRIS has also been used in a study that synthesized and described the photochemical properties of oligo- ortho –azobenzenes.

Other Notes

Effective buffer for the assay of creatine kinase; Effectively protects hemoglobin during freeze-drying; Catholyte in IEF of 2-D gel electrophoresis.
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.
Effective buffer for the assay of creatine kinasecitation; Effectively protects hemoglobin during freeze-drying Catholyte in IEF of 2-D gel electrophoresis.


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Molecules related to Tris and some derivatives that protect haemoglobin during freeze-drying.
P Labrude et al.
The Journal of pharmacy and pharmacology, 32(4), 305-307 (1980-04-01)
J.A.A. Chambers et al.
Electrophoresis, 6, 339-339 (1985)
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because



Global Trade Item Number

SKUGTIN
332550-5G04061826736838
14879-100G-F04061838738059