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46043

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

Synonym(s):

EtDi, EthD-1

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About This Item

Empirical Formula (Hill Notation):
C46H50Cl4N8
CAS Number:
61926-22-5
Molecular Weight:
856.75
NACRES:
NA.32
PubChem Substance ID:
329757072
UNSPSC Code:
12161505
EC Number:
263-325-9
MDL number:
MFCD00210948
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Quality Level

100

description

suitable for the fluorogenic detection of DNA

assay

~90% (HPCE)

form

solid

mol wt

856.75 g/mol

concentration

≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride)

color

red to dark red

mp

≥250 °C (lit.)

solubility

DMF: soluble, DMSO: soluble, H2O: soluble

fluorescence

λex 493 nm in H2O, λex 528 nm; λem 617 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

signalword

Danger

Storage Class

4.3 - Hazardous materials which set free flammable gases upon contact with water

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react. 3

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH3824
BCCF7489
BCCD7665
BCCB5260
BCBX0092

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Global Trade Item Number

SKUGTIN
46043-1MG-F04061832343891
46043-5MG-F04061833277232