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About This Item
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
product line
BioReagent
assay
≥90% (HPLC)
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Application
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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Storage Class
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
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Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 46950-50MG-F | 04061832358949 |
| 46950-1G-F | 04061832358925 |
| 46950-250MG-F | 04061832358932 |