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59304

2-Propanol

Name: 2-Propanol
Brand: SIGMA

≥99.5% (GC), for molecular biology, BioUltra

Synonym(s):

sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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About This Item

Linear Formula:

(CH₃)₂CHOH

CAS Number:

67-63-0

Molecular Weight:

60.10

NACRES:

NA.25

PubChem Substance ID:

57651672

UNSPSC Code:

12352104

EC Number:

200-661-7

MDL number:

MFCD00011674

Beilstein/REAXYS Number:

635639

Assay:

≥99.5% (GC)

Grade:

Molecular Biology

Technique(s):

DNA extraction: suitable, RNA extraction: suitable

Bp:

82 °C (lit.)

Vapor pressure:

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

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Properties

Product Name

2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)

grade

Molecular Biology

Quality Level

200

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

greener alternative product characteristics

Safer Solvents and Auxiliaries
Learn more about the Principles of Green Chemistry.

sustainability

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technique(s)

DNA extraction: suitable, RNA extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

evapn. residue

≤0.001%

color

colorless

refractive index

n20/D 1.377 (lit.), n20/D 1.378

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

SMILES string

CC(C)O

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤0.5 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm A_max: 0.02, λ: 280 nm A_max: 0.01

suitability

suitable for molecular biology

greener alternative category

Aligned

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

Description

General description

2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.

Application

2-Propanol has been used:

to precipitate genomic DNA extracted from flies
to precipitate genomic DNA extracted from xenograft primary tumors and organs
to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)

Biochem/physiol Actions

Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.

Features and Benefits

Suitable as a reagent for Biochemical and Molecular Biology Research
BioUltra grade, meets strict purity standards through rigorous testing
Free from DNase, NICKase, RNase, and Protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This solvent is safe to use and thus aligns with "Safer Solvents and Auxiliaries". Click here for more information.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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pictograms

GHS02,GHS07

signalword

Danger

hcodes

H225,H319,H336

pcodes

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

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Global Trade Item Number

SKU	GTIN
59304-100ML-F	04061832643700
59304-100ML-KC-F	04061838069917
59304-1L-F	04061832643717
59304-500ML-F	04061832643724