Lactic Dehydrogenase, recombinant from E. coli

Name: Lactic Dehydrogenase, recombinant from <I>E. coli</I>
Brand: SIGMA

≥90 U/mg

Synonym(s):

(S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase, Lactate

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About This Item

CAS Number:

9001-60-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-617-8

MDL number:

MFCD00131466

EC Number:

1.1.1.27 (BRENDA, IUBMB)

Specific activity:

≥90 U/mg

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Level

100

form

powder

specific activity

≥90 U/mg

storage temp.

−20°C

Description

General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:

in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
in in vitro DltC D-alanylation assay

Biochem/physiol Actions

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.