Skip to Content
Merck
CN

63689

2-Mercaptoethanol

BioUltra, Molecular Biology, ≥99.0% (GC)

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


grade

Molecular Biology

Quality Level

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.0% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

RNA extraction: suitable, protein extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.500 (lit.), n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.114 g/mL at 25 °C (lit.)

SMILES string

OCCS

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤1 mg/kg, Ni: ≤0.05 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.5, λ: 280 nm Amax: 0.3

storage temp.

2-8°C

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Application

2-Mercaptoethanol has been used
  • in the protein extraction buffer prepared for leaf samples.
  • as a supplement in cell culture media.
  • in the procedure of RNA extraction.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds


Still not finding the right product?

Explore all of our products under 2-Mercaptoethanol


signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品

This item has



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library


Protocols

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Articles

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.


The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans.
Khare S et al.
PLoS ONE, 6, e20850-e20850 (2011)
Adam Healey et al.
Plant methods, 10, 21-21 (2014-07-24)
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction
Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Benitez JJ et al.
Lab on a chip, 12, 4848-4848 (2012)



Global Trade Item Number

SKUGTIN
63689-1ML-KC-F04061838084859
63689-100ML-F04061838357557
63689-25ML-F04061832723167