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Merck
CN

71183

Sodium acetate

anhydrous, BioUltra, suitable for luminescence, Molecular Biology, ≥99.0% (NT)

Synonym(s):

Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt

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About This Item

Linear Formula:
CH3COONa
CAS Number:
Molecular Weight:
82.03
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39021906
UNSPSC Code:
12161700
EC Number:
204-823-8
MDL number:
Beilstein/REAXYS Number:
3595639
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grade

Molecular Biology

Quality Level

product line

BioUltra

assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H2O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg, phosphate (PO43-): ≤5 mg/kg, sulfate (SO42-): ≤20 mg/kg

SMILES string

[Na+].CC([O-])=O

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤10 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤200 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤10 mg/kg, Zn: ≤5 mg/kg

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.02

suitability

suitable for luminescence

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

Application

Sodium acetate is suitable for use as a buffer in:
  • protein crystallization
  • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
  • the preparation of standard (acetylcholine chloride) for hestrin assay
  • substrate screening characterization for microfluidic microbial fuel cell array

Biochem/physiol Actions

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Global Trade Item Number

SKUGTIN
71183-250G04061832843872
71183-1KG04061832843858