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Merck
CN

77671

Anti-Rabbit IgG - Atto 594 antibody produced in goat

Synonym(s):

Atto 594 - [goat-Anti-rabbit IgG]

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
Atto 594 conjugate
Clone:
polyclonal
Application:
Citations:
9
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conjugate

Atto 594 conjugate

Quality Level

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

~1 mg/mL protein

fluorescence

λex 607 nm; λem 626 nm in PBS

suitability

corresponds for gel electrophoresis

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Analysis Note

unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio ≥2

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

常规特殊物品
低风险生物材料

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Articles

免疫印迹(蛋白质印迹转印)是现代蛋白质组学研究中的常用技术。

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.


Lena K Schroeder et al.
The Journal of cell biology, 218(1), 83-96 (2018-11-18)
The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER structure with
Mirko Cortese et al.
Cell host & microbe, 28(6), 853-866 (2020-11-28)
Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human lung epithelial cells.
David Alejandro Bejarano et al.
eLife, 8 (2019-01-24)
Nuclear entry of HIV-1 replication complexes through intact nuclear pore complexes is critical for successful infection. The host protein cleavage-and-polyadenylation-specificity-factor-6 (CPSF6) has been implicated in different stages of early HIV-1 replication. Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-complexes (RTC/PIC)



Global Trade Item Number

SKUGTIN
77671-1ML-F04061838089564