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86826

5-Carboxyfluorescein

Name: 5-Carboxyfluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₂O₇

CAS Number:

76823-03-5

Molecular Weight:

376.32

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57653195

MDL number:

MFCD00036874

Beilstein/REAXYS Number:

57037

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Properties

product line

BioReagent

Quality Level

100

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble, H₂O: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The loss-of-allele assay for ES cell screening and mouse genotyping

Methods in Enzymology, 476, 295-307 (2010)

Detection of Bacteria, Viruses, Parasites and Fungi (2010)

Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes

Hudiyanti D, et al.

Orient. J. Chem., 31, 435-439 (2015)

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Global Trade Item Number

SKU	GTIN
86826-100MG-F	04061832533407
86826-25MG-F	04061833278376