91414
Tris buffered saline with Tween® 20
tablet (for 500 mL), pH 7.6, BioUltra, with Tween® 20
Synonym(s):
TBS, TBST
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Product Name
Tris buffered saline with Tween® 20, BioUltra, tablet (for 500 mL), pH 7.6
product line
BioUltra
Quality Level
form
tablet (for 500 mL)
pH
7.6
solubility
water: 2 tablet/liter, clear, colorless
application(s)
diagnostic assay manufacturing
Application
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris buffered saline with Tween 20, pH 7.6 is used as a washing buffer in assays such as enzyme-linked immunosorbent assays.
Preparation Note
1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Friederike Traunmüller et al.
Journal of immunoassay & immunochemistry, 26(3), 179-188 (2005-07-14)
Immunogenic glycolipids from the cell wall of Mycobacterium tuberculosis are potential capture antigens in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnostis of tuberculosis. Typically, washing steps in ELISAs are performed with buffers containing a detergent. However, Tween-20, the most commonly
T Akimoto et al.
Lupus, 8(9), 761-766 (1999-12-22)
Autoantibodies against prothrombin, including lupus anticoagulant antibodies (LAC), have been identified in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). To identify the epitopes of LAC in patients with SLE and APS, we analyzed B cell epitopes of
Lopa M Das et al.
Autophagy, 15(5), 813-826 (2019-01-22)
Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D enables anti-inflammation to
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 91414-100TAB | 04061833349199 |
| 91414-10TAB | 04061833294307 |