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Merck
CN

94046

Sodium phosphate dibasic solution

BioUltra, 0.5 M in H2O

Synonym(s):

Disodium hydrogen phosphate solution, di-Sodium hydrogen phosphate solution

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25
MDL number:
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product line

BioUltra

Quality Level

form

liquid

concentration

0.5 M in H2O

impurities

insoluble matter, passes filter test, ≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.064 g/mL at 20 °C

anion traces

chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.019, λ: 280 nm Amax: 0.016

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

Application

Sodium phosphate dibasic solution has been used:
  • to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
  • to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
  • to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells

Sodium phosphate dibasic solution may be used in the preparation of a wide variety of phosphate based biological buffers for use in cell and molecular biology application.


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Protocols

在测定醇脱氢酶活性时,该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

View All Protocols

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Megan A Rees et al.
Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have
Jiwei Cui et al.
ACS nano, 9(2), 1571-1580 (2015-02-26)
We report the engineering of poly(ethylene glycol) (PEG) hydrogel particles using a mesoporous silica (MS) templating method via tuning the PEG molecular weight, particle size, and the presence or absence of the template and investigate the cell association and biodistribution



Global Trade Item Number

SKUGTIN
94046-100ML-F04061838170309
94046-500ML-F04061833283745