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Merck
CN

95284

Water

for molecular biology, suitable for FISH

Synonym(s):

H2O

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Grade:
Molecular Biology
Technique(s):
FISH: suitable
Bp:
100 °C (lit.)
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Product Name

Water, Molecular Biology, sterile filtered

grade

Molecular Biology

sterility

sterile-filtered

form

liquid

quality

sterile filtered

shelf life

limited shelf life, expiry date on the label

technique(s)

FISH: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.34 (lit.)

pH

5-7

conductivity

0.0-5 μS/cm at 25 °C

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

cation traces

Ca: <10 ppb, Mg: <10 ppb

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

  • for cloning and plasmid construction.
  • for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.
  • in RNA isolation.
  • fluorescence in situ hybridization (FISH).
  • to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA.
  • for DNA quantification using quantitative polymerase chain reaction (qPCR).


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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia
Fung J, et al.
eLife, 4, e08997-e08997 (2015)
A guide to transient expression of membrane proteins in HEK-293 cells for functional characterization
Ooi A, et al.
Frontiers in Physiology, 7, 300-300 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)



Global Trade Item Number

SKUGTIN
95284-100ML04061837895890
95284-1L04061837895906
95284-6X100ML04061837895913