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Merck
CN

CHP1

Imprint® Chromatin Immunoprecipitation Kit

Complete ChIP reaction in 6 hours in flexible strip well format

Synonym(s):

Chromatin Immunoprecipitation

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About This Item

NACRES:
NA.54
UNSPSC Code:
41116158
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Quality Level

storage temp.

−20°C

General description

Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.

For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.

Application

The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
  • Sensitive—As few as 10,000 cells required for each ChIP sample
  • Convenient—Fewest steps of any available ChIP protocol
  • Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
  • Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
GenomePlex is a registered trademark of Takara Bio USA, Inc.


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • Column Preparation Solution
    SDS

  • Proteinase K from Tritirachium album, buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL
    SDS

  • Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
    SDS

  • Normal Mouse IgG antibody produced in mouse
    SDS

  • Anti-RNA polymerase II from mouse
    SDS

signalword

Danger

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Regulatory Information

监管及禁止进口产品

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Protocols

Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统,可实现快速的高通量ChIP检测

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format


Ghazala Begum et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
Undernutrition during pregnancy is implicated in the programming of offspring for the development of obesity and diabetes. We hypothesized that maternal programming causes epigenetic changes in fetal hypothalamic pathways regulating metabolism. This study used sheep to examine the effect of
Lucie Hyrsova et al.
British journal of pharmacology, 173(10), 1703-1715 (2016-02-28)
The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters
Thomas Palm et al.
Nucleic acids research, 41(6), 3699-3712 (2013-02-12)
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and



Global Trade Item Number

SKUGTIN
APREST71098-100UL04061835939237
HPA006616-100UL04061835679980
HPA006616-25UL04061842777327
CHP1-24RXN04061833531815
CHP1-96RXN04061833531822
EHU110131-20UG04061828593064
EHU110131-50UG04061828356270