A2512
Albumin from chicken egg white
lyophilized powder, ≥98% (agarose gel electrophoresis)
Synonym(s):
Albumin from hen egg white, Ovalbumin
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About This Item
CAS Number:
UNSPSC Code:
12352202
eCl@ss:
42010201
EC Number:
232-692-7
NACRES:
NA.61
MDL number:
Biological source:
chicken egg white
Assay:
≥98% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
bioconjugation: suitable
Concentration:
4—20 mol/mol albumin
biological source
chicken egg white
Quality Level
type
Grade VI
assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
44287 Da by calculation
purified by
chromatography
concentration
4—20 mol/mol albumin
extent of labeling
4-20 mol mannose per mol ovalbumin protein
technique(s)
bioconjugation: suitable
solubility
H2O: soluble 50 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... OVAL(396058)
General description
Ovalbumin makes 75% of the total egg white protein.
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.
Application
Ovalbumin has been used:
- as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil
- to induce allergic inflammation and atopic dermatitis in experimental mice
- as a component in control mixture for validating tandem mass spectral analysis
- as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Biochem/physiol Actions
Albumin from chicken egg white serves as a natural adhesive.
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.
Physical form
Essentially salt free
Preparation Note
A further purification of A 5503 to reduce mannose content.
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signalword
Danger
hcodes
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
pcodes
Hazard Classifications
Resp. Sens. 1
Regulatory Information
动物来源生物产品
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Related Content
PTP1B deficiency exacerbates inflammation and accelerates leukocyte trafficking in vivo
<BIG>Berdnikovs S, et al.</BIG>
Journal of Immunology, 188, 874-884 (2012)
Experimental protein mixture for validating tandem mass spectral analysis
<BIG>Keller A, et al.</BIG>
Omics : A Journal of Integrative Biology, 6, 207-212 (2002)
In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines
<BIG>Heffernan MJ, et al.</BIG>
Biomaterials, 32, 926-932 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A2512-250MG | 04061833350379 |
| A2512-1G | 04061833350362 |
| A2512-5G | 04061833350386 |