Anti-ATG4B antibody produced in rabbit

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.

ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Anti-ATG4B antibody produced in rabbit has been used in

• western blotting
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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