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Merck
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A3157

Alcian Blue 8GX

Dye content, ≥50%, certified by the Biological Stain Commission

Synonym(s):

Alcian Blue, Ingrain Blue 1

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About This Item

Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
251-705-7
MDL number:
Colour Index Number:
74240
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Product Name

Alcian Blue 8GX, powder or crystals, certified by the BSC

agency

certified by the BSC

Quality Level

form

powder or crystals

composition

Dye content, ≥50%

technique(s)

microbe id | staining: suitable

color

dark blue

solubility

water: 1 mg/mL, blue

εmax

195-400 at 617-630 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.
Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Articles

为满足蛋白分析的多样化需求,Sigma提供多种蛋白质可视化(染色)试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计,即使在传统的PAGE形式中依然表现出色。

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.


Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method.
Sun S, et al.
Stem Cells, 21, 527-535 (2003)
Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation.
Alves da Silva ML, et al.
Acta Biomaterialia, 6, 1149-1157 (2010)
Lara Vlietstra et al.
PloS one, 14(6), e0217462-e0217462 (2019-06-07)
To determine if there is an association between sarcopenia, physical function and self-reported fatigue in osteoarthritis (OA) and rheumatoid arthritis (RA). A cross-sectional analysis of measurements from a cohort of 157 participants with OA or RA was performed. The relationship



Global Trade Item Number

SKUGTIN
A3157-10G04061835570959
A3157-25G04061835570966