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Merck
CN

A4418

Ammonium sulfate

Molecular Biology, ≥99.0%

Synonym(s):

Mascagnite

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About This Item

Linear Formula:
(NH4)2SO4
CAS Number:
Molecular Weight:
132.14
PubChem Substance ID:
eCl@ss:
38030408
UNSPSC Code:
12352314
NACRES:
NA.31
EC Number:
231-984-1
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 0.667 g/mL
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grade

Molecular Biology

Quality Level

assay

≥99.0%

form

powder

technique(s)

affinity binding assay: suitable, protein purification: suitable

impurities

≤0.005% Insolubles

pH

5.0-6 (25 °C, 132 g/L)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 0.667 g/mL

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤5 ppm, Fe: ≤5 ppm, Zn: ≤5 ppm, heavy metals (as Pb): ≤2 ppm

absorption

≤0.025 at 280 at 1 M, ≤0.03 at 260 at 1 M

suitability

suitable for flow cytometry, suitable for molecular biology

application(s)

metabolomics
microbiology

foreign activity

DNase, RNase, and protease, none detected

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

General description

Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins. Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media. Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.

Application

Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used
  • in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins.
  • to precipitate the Shiga-like toxin from the culture supernatant.
  • for precipitaion of viral particles from the culture supernatant.

Features and Benefits

  • Heavy metals (as Pb): ≤2 ppm
  • Free from DNase, RNase, and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
Petia Kovatcheva-Datchary et al.
Cell reports, 26(13), 3772-3783 (2019-03-28)
The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To
James F Hunter et al.
Environmental science & technology, 48(17), 10227-10234 (2014-08-06)
A large number of organic species emitted into the atmosphere contain cycloalkyl groups. While cyclic species are believed to be important secondary organic aerosol (SOA) precursors, the specific role of cyclic moieties (particularly for species with multiple or fused rings)



Global Trade Item Number

SKUGTIN
A4418-100G04061835542093
A4418-1KG04061835542109
A4418-500G04061835542116
A4418-5KG04061833374474