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A6279

Aprotinin

3-7 TIU/mg protein, saline solution

Synonym(s):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

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About This Item

Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
9087-70-1
Molecular Weight:
6511.44
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00130541
eCl@ss:
32190102
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Product Name

Aprotinin from bovine lung, saline solution, 3-7 TIU/mg protein

biological source

bovine

Quality Level

300

sterility

aseptically filled

form

saline solution

specific activity

3-7 TIU/mg protein

mol wt

~6,500

solubility

H2O: soluble >10 mg/mL

UniProt accession no.

P00974

storage temp.

2-8°C

SMILES string

S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O

InChI

1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Gene Information

cow ... PTI(404172)

General description

Aprotinin is a 58 amino acid-long protein, with a calculated molecular weight of 6512 Da, that acts as a serine protease inhibitor. Known also as bovine pancreatic trypsin inhibitor (BPTI), aprotinin inhibits serine proteases such as chymotrypsin, kallikrein, plasmin and trypsin.

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Preparation Note

This product is an aseptically filled solution in 0.9% NaCl and 0.9% benzyl alcohol. It has an activity of 3-7 TIU/mg protein and 3-21 TIU/mL solution.

Other Notes

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

Disclaimer

Aprotinin products should be stored at 2-8°C.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

动植物源性产品
低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ9493
SLCG5705
SLCC9045
SLBZ9668
SLBX1758

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Protocols

抑肽酶酶活测定

抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Association between use of antenatal magnesium sulfate in preterm labor and adverse health outcomes in infants.
Robert Mittendorf et al.
American journal of obstetrics and gynecology, 189(2), 613-613 (2003-10-04)
Kazuya Machida et al.
PloS one, 5(10), e13470-e13470 (2010-10-27)
Tyrosine kinases drive the proliferation and survival of many human cancers. Thus profiling the global state of tyrosine phosphorylation of a tumor is likely to provide a wealth of information that can be used to classify tumors for prognosis and
Molecular basis for p38 protein kinase inhibitor specificity
Lisnock et al.
Biochemistry, 38(11), 3456-3456 (1999-03-17)
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Global Trade Item Number

SKUGTIN
A6279-5X10ML04061833381984
A6279-10ML04061838251596
A6279-50ML04061833381960
A6279-5ML04061833381977