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Merck
CN

A7979

Bovine Serum Albumin solution

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
33-37% total protein basis (UV)
Form:
liquid
Technique(s):
blocking: suitable, cell culture | mammalian: suitable
Impurities:
≤1000 EU/mL Endotoxin level
Concentration:
35% in DPBS
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biological source

bovine

Quality Level

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

assay

33-37% total protein basis (UV)

form

liquid

origin

USA origin

concentration

35% in DPBS

technique(s)

blocking: suitable, cell culture | mammalian: suitable

impurities

≤1000 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile On raw material, none detected

shipped in

ambient

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.


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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

动物来源生物产品

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Articles

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.


Neil T Burford et al.
Assay and drug development technologies, 15(7), 320-329 (2017-11-10)
Homogeneous functional assays that utilize competition binding technology are widely used for determining pharmacological properties such as intrinsic activity and potency. One example is time-resolved fluorescence resonance energy transfer (TR-FRET) 3',5'-cyclic adenosine monophosphate (cAMP) assays, where labeled cAMP (tracer) and
Gustavo A Gomez et al.
Developmental biology, 449(2), 99-106 (2019-03-04)
The developmental biology of neural crest cells in humans remains unexplored due to technical and ethical challenges. The use of pluripotent stem cells to model human neural crest development has gained momentum. We recently introduced a rapid chemically defined approach
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in



Global Trade Item Number

SKUGTIN
A7979-12X50ML04061833390856
A7979-50ML04061833390863