form
aqueous suspension
Quality Level
extent of labeling
40-80 μmol per mL
matrix
6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
through amino to carboxyls of EDTA
matrix spacer
9 atoms
capacity
≥8 mg/mL binding capacity (peroxidase Type VI)
storage temp.
2-8°C
SMILES string
[X]Nc1cc(ccc1)B(O)O
General description
m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.
Application
m-Aminophenylboronic acid-Agarose has been used:
- as a component of the column for the purification of Escherichia coli TOP10F cells
- with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
- in the affinity purification of horseradish peroxidase (HRPO)
- in the glycated human serum albumin (gHSA) purification
Physical form
Suspension in water containing 0.002% chlorhexidine diacetate.
Still not finding the right product?
Explore all of our products under m-Aminophenylboronic acid–Agarose
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Glycobiology and Glycoproteomics Brochure
T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A8312-5ML | 04061833391426 |