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Merck
CN

A8332

p-Aminobenzamidine–Agarose

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

Synonym(s):

pABA

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.56
MDL number:
Form:
(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)
Biological source:
plant
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biological source

plant

Quality Level

form

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

technique(s)

protein purification: suitable

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino to carboxyl of spacer

matrix spacer

9 atoms

capacity

≥5 mg/mL binding capacity (trypsin)

storage temp.

2-8°C

Application

p-Aminobenzamidine Agarose (pABA) can be used as an affinity ligand to bind and remove serine proteases in addition to Ca2+-binding proteins. It has also been used in medical applications as a tool for the purification of non-protease proteins.

Physical form

1:1 suspension in a 1.0 M NaCl + 20% ethanol solution


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Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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Paola Fortugno et al.
Journal of cell science, 115(Pt 3), 575-585 (2002-02-28)
Survivin is a member of the inhibitor of apoptosis gene family that has been implicated in both apoptosis inhibition and regulation of mitosis. However, the subcellular distribution of survivin has been controversial and variously described as a microtubule-associated protein or
Massimo D'Agostino et al.
Nature, 551(7682), 634-638 (2017-11-02)
Membrane fusion in eukaryotic cells mediates the biogenesis of organelles, vesicular traffic between them, and exo- and endocytosis of important signalling molecules, such as hormones and neurotransmitters. Distinct tasks in intracellular membrane fusion have been assigned to conserved protein systems.
Tomas Erban
PloS one, 6(8), e22860-e22860 (2011-08-31)
p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification



Global Trade Item Number

SKUGTIN
A8332-25ML04061837769627
A8332-10ML04061837769610
A8332-5ML04061837769634