A8706
Avidin from egg white
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Synonym(s):
AVID_CHICK
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Form:
powder
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in corn
Mol wt:
glycoprotein 66 kDa, subunit 16 kDa
recombinant
expressed in corn
Quality Level
assay
≥80% protein basis (biuret)
form
powder
specific activity
≥12 units/mg protein (E1%/280)
mol wt
glycoprotein 66 kDa, subunit 16 kDa
technique(s)
ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... AVD(396260)
General description
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Application
Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
Preparation Note
Affinity purified
Other Notes
One unit will bind 1.0 μg of d-biotin.
Still not finding the right product?
Explore all of our products under Avidin from egg white
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Timothy R J Holford et al.
Biosensors & bioelectronics, 44, 198-203 (2013-02-23)
We describe within this paper the construction of a label-free immunosensor for the protein psoriasin (S100A7), which is associated with a number of clinical conditions such as skin diseases or cancer. Antibodies to psoriasin were immobilised onto screen-printed carbon electrodes
M J Navakouskiĭ et al.
Bioorganicheskaia khimiia, 38(4), 449-457 (2012-11-30)
A new construction of a test-system for free thyroid hormone 3,3',5-triiodothyronine determination (free T3) in human blood serum in ELISA and IRMA variants was described. For this purpose a low-molecular weight bifunctional conjugate containing T3 and vitamin H (biotin, Bt)
Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A8706-5MG | 04061833400562 |