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A8717

Anti-Amyloid Precursor Protein (APP) Antibody

rabbit polyclonal

Synonym(s):

APP antibody, Anti-APP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02095828
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
310
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Product Name

Anti-Amyloid Precursor Protein, C-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 95-100 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, microarray: suitable, western blot: 1:4,000 using rat brain extract

UniProt accession no.

P05067

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APP(351)
mouse ... App(11820)
rat ... App(54226)

General description

Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APP is expressed in the brain. It is located on human chromosome 21. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. Accumulation of the cleavage products of APP, such as the β-amyloid peptide, can cause Alzheimer′s disease.
The immunogen sequence is identical to rat and mouse APP695 and in the APP isoforms APP751 and APP770. The antibody recognizes APP695, APP751 and APP770.

Immunogen

Synthetic peptide corresponding to the C-terminal of human APP695 conjugated to KLH.

Application

Rabbit Anti-Amyloid Precursor Protein, C-Terminal antibody has been used for immunoprecipitation, immunohistochemical analysis, immunohistochemical staining and western blot applications. The product can also be used for IHC (1:1,000) and microarray studies.
SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.

Biochem/physiol Actions

In transiently transfected cell lines, APP (amyloid precursor protein) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP acts as a transcriptional regulator and its amino acid domain serves as a regulator of its own intracellular sorting. Mutation of the gene leads to cerebral amyloid angiopathy. Accumulation of the cleavage products of APP, such as the β-amyloid peptide can cause Alzheimer′s disease.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

Regulatory Information

低风险生物材料
常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
099M4786V
21190422
049M4882V
078M4776V
107M4816V

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Related Content

Fang Cheng et al.
The Journal of biological chemistry, 286(31), 27559-27572 (2011-06-07)
Amyloid β (Aβ) is generated from the copper- and heparan sulfate (HS)-binding amyloid precursor protein (APP) by proteolytic processing. APP supports S-nitrosylation of the HS proteoglycan glypican-1 (Gpc-1). In the presence of ascorbate, there is NO-catalyzed release of anhydromannose (anMan)-containing
Amyloid Precursor Protein Processing and Alzheimer?s Disease.
Richard J O, et al.
Annual Review of Neuroscience, 34, 185-204 (2001)
Novel amyloid precursor protein mutation in an Iowa family with dementia and severe cerebral amyloid angiopathy
Grabowski TJ, et al.
Annals of Neurology, 49(6), 697-705 (2001)
View All Related Papers

Global Trade Item Number

SKUGTIN
A8717-.2ML04061833400579
A8717-100UL04061838029751
A8717-25UL04061838448163