A9941
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
chromogenic, tablet
Synonym(s):
AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
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About This Item
Product Name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, tablet, 10 mg substrate per tablet
Quality Level
form
tablet
solubility
deionized water: 100 mL
storage temp.
room temp
SMILES string
[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI
1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
General description
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:
- For a reaction with laccase or bilirubin oxidase
- For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound
Application
- 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
- ABTS is also used to measure glucose concentrations in different solutions including blood serum.
- The food industry uses ABTS to measure the antioxidant capacities within different foods.
- Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
- It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Preparation Note
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
wgk
WGK 2
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
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Protocols
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
Articles
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9941-100TAB | 04061833418291 |
| A9941-50TAB | 04061833418307 |
| A9941-4TAB | 04061831168501 |
| A9941-5TAB | 04061833418314 |