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About This Item
Conjugate:
unconjugated
Clone:
BU-33, monoclonal
Application:
ICC, IHC (p)
Citations:
214
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BU-33, monoclonal
species reactivity
wide range
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using sections of intestine from mouse or rat treated in vivo with BrdU
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH.
Immunogen
bromodeoxyuridine conjugated to KLH.
Application
Monoclonal Anti-BrdU antibody has been used in immunostaining, BrdU incorporation into DNA, immunohistochemistry and uterine labeling index. It has also been used to investigate cell proliferation.
Biochem/physiol Actions
5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.
Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.
Preparation Note
For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Other Notes
This product can be found as purified product that was produced using cell culture hybridoma product.
B8434 Anti-BrdU antibody, Mouse monoclonal
clone BU-33, purified from hybridoma cell culture
B8434 Anti-BrdU antibody, Mouse monoclonal
clone BU-33, purified from hybridoma cell culture
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
动物来源生物产品
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Related Content
Alvaro Duque et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(42), 15205-15217 (2011-10-22)
As markers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system. However, the effect of the incorporation of
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B2531-.2ML | 04061833426890 |
| B2531-100UL | 04061833426906 |