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Merck
CN

B3111

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

Synonym(s):

Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Species reactivity:
-
Application:
Citations:
2
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biological source

mouse

antibody form

purified immunoglobulin (purified IgG1 subclass)

clone

M2, monoclonal

shelf life

4 yr

purified by

using Protein A

storage temp.

−20°C

General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG® application portal.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
常规特殊物品

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Silvia Martini et al.
Nature communications, 12(1), 6934-6934 (2021-11-28)
The PKCε-regulated genome protective pathway provides transformed cells a failsafe to successfully complete mitosis. Despite the necessary role for Aurora B in this programme, it is unclear whether its requirement is sufficient or if other PKCε cell cycle targets are
Romain Forey et al.
Molecular cell, 81(1), 183-197 (2020-12-06)
Mre11-Rad50-Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin



Global Trade Item Number

SKUGTIN
B3111-1MG04061841248514
B3111-0.2MG04061841248507