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B3279

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Name: <I>N</I><SUB>&#945;</SUB>-Benzoyl-<SC>L</SC>-arginine 4-nitroanilide hydrochloride
Brand: SIGMA

proteolytic enzyme substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

L-BAPA, L-BAPNA, BANI

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₂₂N₆O₄ · HCl

CAS Number:

21653-40-7

Molecular Weight:

434.88

NACRES:

NA.32

PubChem Substance ID:

24891697

UNSPSC Code:

12352204

EC Number:

244-505-6

MDL number:

MFCD00063682

Beilstein/REAXYS Number:

4081878

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Properties

Product Name

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥98% (HPLC)

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI key

DEOKFPFLXFNAON-NTISSMGPSA-N

Description

General description

Chromogenic substrate for trypsin and other proteolytic enzymes.

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:

to measure papain activity in spectrophotometric amidase assay
to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
for trypsin

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
B3279-100MG	04061833427569
B3279-1G	04061833427576
B3279-250MG	04061833427583