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B4387

5-Bromo-3-indolyl β-D-galactopyranoside

Name: 5-Bromo-3-indolyl &#946;-<SC>D</SC>-galactopyranoside
Brand: SIGMA

≥98%, powder

Synonym(s):

Bluo-Gal

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₆BrNO₆

CAS Number:

97753-82-7

Molecular Weight:

374.18

NACRES:

NA.83

PubChem Substance ID:

24891771

UNSPSC Code:

12352204

MDL number:

MFCD00063691

Beilstein/REAXYS Number:

1550140

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Properties

Product Name

5-Bromo-3-indolyl β-D-galactopyranoside, ≥98%

Quality Level

200

assay

≥98%

form

powder

solubility

DMF: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

InChI key

LINMATFDVHBYOS-MBJXGIAVSA-N

Description

Application

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品

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[Determination of beta-galactosidase activity on nitrocellulose plates using 5-bromo-3-indolyl-beta-D-galactopyranoside and tetrazolium salts].

A N Markarian et al.

Bioorganicheskaia khimiia, 13(2), 263-265 (1987-02-01)

A simple and convenient technique has been developed for detection of beta-galactosidase from E. coli on nitrocellulose sheets using a mixture of 5-bromoindol-3-yl-beta-D-galactopyranoside and nitro blue tetrazolium, which enables rapid detection of fmole (10(-15) mole) quantities of the enzyme at

Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

H Kodaka et al.

Journal of clinical microbiology, 33(1), 199-201 (1995-01-01)

A new medium containing 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (Glu agar) for Escherichia coli and a new medium containing 5-bromo-3-indolyl-beta-D-galactoside (Gal agar) for beta-galactosidase-positive members of the family Enterobacteriaceae were compared with MacConkey agar in a diagnostic trial with 3,562 urine specimens.

Cellular transformation of mouse embryo fibroblasts in the absence of activator E2Fs.

Tushar Gupta et al.

Journal of virology, 89(9), 5124-5133 (2015-02-27)

The E2F family of transcription factors, broadly divided into activator and repressor E2Fs, regulates cell cycle genes. Current models indicate that activator E2Fs are necessary for cell cycle progression and tumorigenesis and are also required to mediate transformation induced by

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Global Trade Item Number

SKU	GTIN
B4387-100MG	04061833258675
B4387-25MG	04061833429624