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B5002

5-Bromo-2′-deoxyuridine

Name: 5-Bromo-2&#8242;-deoxyuridine
Brand: SIGMA

≥99% (HPLC), synthetic (organic), powder

Synonym(s):

5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

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About This Item

Empirical Formula (Hill Notation):

C₉H₁₁BrN₂O₅

CAS Number:

59-14-3

Molecular Weight:

307.10

NACRES:

NA.51

PubChem Substance ID:

24278270

UNSPSC Code:

41106305

EC Number:

200-415-9

MDL number:

MFCD00006529

Beilstein/REAXYS Number:

30395

Assay:

≥99% (HPLC)

Biological source:

synthetic (organic)

Form:

powder

Solubility:

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

Storage temp.:

−20°C

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Properties

Product Name

5-Bromo-2′-deoxyuridine, ≥99% (HPLC)

biological source

synthetic (organic)

Quality Level

200

assay

≥99% (HPLC)

form

powder

mp

191-194 °C (dec.) (lit.)

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

Description

Application

5-Bromo-2′-deoxyuridine has been used:

as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis
as a supplement to study the loss-of-gene function in adult zebrafish heart
in T lymphocyte proliferation assay{133]

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

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pictograms

GHS08

signalword

Danger

hcodes

H340,H361fd

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Muta. 1B - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Cell Cycle Analysis Using NTP Transporter

Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.

Global Trade Item Number

SKU	GTIN
B5002-100G	04061835517671
B5002-100MG	04061835553860
B5002-5G	04061835553907
B5002-1G	04061835553877
B5002-250MG	04061835553884
B5002-500MG	04061835553891