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Merck
CN

B8049

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt

tablet

Synonym(s):

X-GlcA, X-glucuronide CHA salt

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About This Item

Empirical Formula (Hill Notation):
C14H13BrClNO7 · C6H13N
CAS Number:
Molecular Weight:
521.79
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
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form

tablet

Quality Level

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI key

JXCKZXHCJOVIAV-CYRSAHDMSA-N

Application

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.
Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by Escherichia coli.

Preparation Note

Contains 10 mg substrate per tablet.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Global Trade Item Number

SKUGTIN
B8049-5TAB04061833442456