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B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%, powder, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
NACRES:
NA.83
PubChem Substance ID:
57653968
UNSPSC Code:
12352204
EC Number:
230-640-8
MDL number:
MFCD00005666
Beilstein/REAXYS Number:
1402009
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Product Name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

Quality Level

200

assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL8552
SLCK8733
SLCJ4711
SLCH7106
SLCD7060

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Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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Articles

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感受态细胞生长及其转化（吸收DNA）的通用实验方案。

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转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

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General protocols for growth of competent cells and their transformation (uptake of DNA).

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Global Trade Item Number

SKUGTIN
B9146-100MG04061835553976
B9146-10MG04061835540716
B9146-500MG04061835511846