Key Documents

View All Documentation

B9385

Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride

Name: Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride
Brand: SIGMA

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₃₁H₄₅N₇O₇

CAS Number:

70375-24-5

Molecular Weight:

627.73

NACRES:

NA.32

PubChem Substance ID:

24892134

UNSPSC Code:

12352204

MDL number:

MFCD00058181

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

assay

≥98% (TLC)

Quality Level

200

form

powder

solubility

water: 20 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

Cl.CC(C)C(NC(=O)OC(C)(C)C)C(=O)N1CCCC1C(=O)NC(CCCNC(N)=N)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C31H45N7O7.ClH/c1-17(2)25(37-30(43)45-31(4,5)6)28(42)38-14-8-10-22(38)27(41)36-21(9-7-13-34-29(32)33)26(40)35-19-11-12-20-18(3)15-24(39)44-23(20)16-19;/h11-12,15-17,21-22,25H,7-10,13-14H2,1-6H3,(H,35,40)(H,36,41)(H,37,43)(H4,32,33,34);1H

InChI key

ROQGJTZGLVLVIN-UHFFFAOYSA-N

Description

General description

A fluorogenic substrate for thrombin.

Boc-Val-Pro-Arg-AMC serves as a synthetic substrate for human kallikrein 8.

Application

Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride has been used:

as a fluorogenic substrate for thrombin in inhibitory assay with salivary protein cE5
as a fluorogenic substrate for thrombin in inhibitory assay with recombinant E. nipponicum serpin
as a substrate in proteinase assay with hemolymph protein

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Activation and enzymatic characterization of recombinant human kallikrein 8.

Tadaaki Kishi et al.

Biological chemistry, 387(6), 723-731 (2006-06-28)

Human kallikrein 8 (hK8), whose gene was originally cloned as the human ortholog of a mouse brain protease, is known to be associated with diseases such as ovarian cancer and Alzheimer's disease. Recombinant human pro-kallikrein 8 was activated with lysyl

Functional analyses yield detailed insight into the mechanism of thrombin inhibition by the antihemostatic salivary protein cE5 from Anopheles gambiae.

Luciano Pirone et al.

The Journal of biological chemistry, 292(30), 12632-12642 (2017-06-09)

Saliva of blood-feeding arthropods carries several antihemostatic compounds whose physiological role is to facilitate successful acquisition of blood. The identification of novel natural anticoagulants and the understanding of their mechanism of action may offer opportunities for designing new antithrombotics disrupting

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

T Morita et al.

Journal of biochemistry, 82(5), 1495-1498 (1977-11-01)

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were

View All Related Papers

Global Trade Item Number

SKU	GTIN
B9385-5MG	04061833443736