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C0775

Crystal Violet

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonym(s):

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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About This Item

Linear Formula:
C25H30N3Cl
CAS Number:
548-62-9
Molecular Weight:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57653980
EC Number:
208-953-6
MDL number:
MFCD00011750
Beilstein/REAXYS Number:
3580948
Colour Index Number:
42555
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Product Name

Crystal Violet, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

2.5 — 3.5 (20 °C)

mp

205 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

density

1.19 g/cm3 at 20 °C

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes, enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.
Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM1562
SHBL8391
SHBL6131
MKCG7859
MKCG3071

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Sharma A
Plant Chromosomes (1999)
Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model.
Liao CJ, et al.
Reproduction (Cambridge, England), 147, 179-179 (2013)
Carson S and Robertson D
Manipulation and Expression of Recombinant DNA (2005)
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Global Trade Item Number

SKUGTIN
C0775-100G04061833452080
C0775-25G04061833452097