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C3306

Calcium chloride dihydrate

Name: Calcium chloride dihydrate
Brand: SIGMA

Molecular Biology, ≥99.0%

Synonym(s):

CaCl₂.2H₂O, Calcium chloride, Calcium dichloride

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About This Item

Empirical Formula (Hill Notation):

CaCl₂ · 2H₂O

CAS Number:

10035-04-8

Molecular Weight:

147.01

NACRES:

NA.31

PubChem Substance ID:

24892603

UNSPSC Code:

12352302

EC Number:

233-140-8

MDL number:

MFCD00149613

Assay:

≥99.0%

Grade:

Molecular Biology

Form:

powder

Solubility:

H₂O: 294 mg/mL, clear, colorless

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Properties

grade

Molecular Biology

Quality Level

200

assay

≥99.0%

form

powder

mp

176 °C (dec.) (lit.)

solubility

H₂O: 294 mg/mL, clear, colorless

foreign activity

DNase, RNase, protease, none detected

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Description

Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

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pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Preparation of calcium competent Escherichia coli and heat-shock transformation

Chang AY, et al.

Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)

Glucagon-Like Peptide-2 and the Enteric Nervous System Are Components of Cell-Cell Communication Pathway Regulating Intestinal Na+/Glucose Co-transport.

Andrew W Moran et al.

Frontiers in nutrition, 5, 101-101 (2018-11-13)

The Na+/glucose cotransporter 1, SGLT1 is the major route for transport of dietary glucose from the lumen of the intestine into absorptive enterocytes. Sensing of dietary sugars and artificial sweeteners by the sweet taste receptor, T1R2-T1R3, expressed in the enteroendocrine

Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis.

Yujin E Kim et al.

Nature protocols, 5(8), 1396-1405 (2010-07-31)

Applications for noninvasive real-time imaging of apoptosis and neuronal degeneration are hindered by technical limitations in imaging strategies and by existing probes. Monitoring the progression of a cell through apoptosis could provide valuable insight into the temporal events that initiate

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Global Trade Item Number

SKU	GTIN
C3306-500G	04061833485729
C3306-100G	04061833485705
C3306-250G	04061833485712