CA200
cAMP Enzyme Immunoassay Kit, Direct
Synonym(s):
cAMP detection kit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Technique(s):
ELISA: suitable
Species reactivity:
-
Application:
ELISA
Citations:
23
General description
Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.
Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.
Application
Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium.
Biochem/physiol Actions
Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.
Other Notes
Allow reagents to warm at room temperature for 30 minutes prior to opening.
Kit Components Only
Product No.
Description
- 0.1 M Hydrochloric Acid 1 x 30
- Acetic Anhydride 1 x 1
- cAMP-Alkaline Phosphatase Conjugate 1 x 5
- cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5
- 5 Cycle Logit-Log Paper 1 x 1
- Cyclic AMP Standard 1 x 0.5
- Direct cAMP Assay Layout Sheet 1 x 1
See All (14)
signalword
Danger
target_organs
Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
120.2 °F
flash_point_c
49 °C
wgk
WGK 3
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Regulatory Information
危险化学品
常规特殊物品
低风险生物材料
易制毒化学品（2类）
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
María Inés Marchesini et al.
Cellular microbiology, 13(8), 1261-1274 (2011-06-29)
Type IV secretion systems (T4SS) are specialized protein complexes used by many bacterial pathogens for the delivery of effector molecules that subvert varied host cellular processes. Brucella spp. are facultative intracellular pathogens capable of survival and replication inside mammalian cells.
Haemodynamic and extracellular matrix cues regulate the mechanical phenotype and stiffness of aortic endothelial cells.
Collins C, Osborne LD, Guilluy C, et al.
Nature Communications, 5, 3984-3984 (2014)
Anna Maria D'Ursi et al.
Molecular pharmacology, 69(3), 727-736 (2005-12-08)
Cell-penetrating peptides are able to transport covalently attached cargoes such as peptide or polypeptide fragments of endogenous proteins across cell membranes. Taking advantage of the cell-penetrating properties of the 16-residue fragment penetratin, we synthesized a chimeric peptide that possesses an
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CA200-1KT | 04061833531457 |