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Jurkat E6.1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88042803, human blood (leukemic T-cell lymphoblast), Not specified

Synonym(s):

Jurkat Cells, Jurkat Clone E6-1 Cells

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About This Item

UNSPSC Code:
41106514
Biological source:
human blood (leukemic T-cell lymphoblast)
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Pseudodiploid, modal No 46
Morphology:
Not specified
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biological source

human blood (leukemic T-cell lymphoblast)

packaging

tube of 5 μg 88042803-DNA-5UG, pkg of vial of cells 88042803-1VL

growth mode

Suspension

karyotype

Pseudodiploid, modal No 46

morphology

Not specified

products

Interleukin 2 (IL-2), human alpha interferon

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro
IL-2 assay and cloning studies

Biochem/physiol Actions

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Human leukaemic T cell lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

常规特殊物品
低风险生物材料

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Protocols

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Articles

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.